Advantages of MES in aseptic culture: According to the results of MES buffer inhibiting the generation of superoxide in the root tips and affecting the root zone and growth of Arabidopsis, 0.01% and 0.1% of MES promote root growth from the early development stage of seedlings, while 1% of MES is inhibited. 2.1-2mMMES solution has excellent buffering ability and does not inhibit nodulation or reduce nitrogen fixation in soybean hydroponic culture. 5mMMES has no effect on the growth or absorption of most nutrients, and MES buffer can even enhance the potassium absorption of soybean seedlings without nodules. 3. In the study of MES, it was found that the combination of 10mgl-1 arabic gum and 3mMMES in NLN-13 significantly improved the efficiency of microspore embryogenesis in cabbage microspore culture under the influence of arabinogalactan protein at pH 6.4. In NH4 nutrition research, it was found that the addition of MES significantly increased plant yield and reduced node number at all concentrations; In the study on the effect of pH value on the rooting of apple micro bud stem segments, it was found that when MES was used as a buffer, the pH value was stable between 5.0-6.5. During the growth process of rice suspension culture, the addition of MES and proline in the culture medium has a significant impact on cell proliferation; After adding 500mgl-1 of PVP, casein hydrolysate, and MES buffer to the culture medium, a large amount of callus tissue was observed. In the study of early nodulation of annual alfalfa using MES buffer, it was found that a 0.5mM MES solution can stabilize the pH value by daily titration and using a high nitrate to ammonium ratio (9:1) in the solution. When used in MES for autotrophic micropropagation, the results showed that a fertilizer solution containing 5-mMMES buffer per 300mL container stabilized the pH value of the culture medium and improved the success rate of rooting.