What is TRICINE buffer TRICINE Buffer (CAS 5704-04-1) is a zwitterionic biological buffer containing amino and acid groups. This product is a white crystalline powder, slightly soluble in water (solubility of 1.8g/100ml water). The Application and Advantages of TRICINE Buffer TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of Mycoplasma in animal tissue culture. It has also been used in bacterial culture media to prevent iron salt precipitation. The Good's buffer solution can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni and Zn. TRICINE has higher negative (more negative) charges than glycine, which makes its migration speed faster. Its high ionic strength leads to more ion movement and less protein movement, so that low molecular weight proteins can be separated in a lower percentage of acrylamide gel. Experience in using Tricine-SDS-PAGE adhesive In experiments, it is common to encounter target proteins that need to be expressed below 10kd. If conventional SDS-PAGE is used to run small molecule proteins, it can cause blurriness, soft glia, and even inability to run out. Tricine gel can effectively solve the problem of identifying small molecule proteins below 10kd. Tricine glue is relatively easy to flatten the sample, and this type of glue produces thin and straight markers. Similarly, the visible bands are also well pressed. Compared with the results obtained by ordinary SDS-PAGE glue, the diffusion of the Marker is not as severe, and the spacing between the stripes in each lane is obvious, unlike the stripes of ordinary glue that are easily connected together. Tris Tricine SDS Precautions During the Tricine-SDS-PAGE process, anode and cathode buffer solutions were used, with the anode buffer solution being Tris-HCl 200 mM and pH 8.9; Cathode buffer: Tris 100 mM; Tricine 100 mM; SDS 0.1%, pH 8.3. Product advantage: It is an ideal method for separating small molecule peptides. Note 1: If the TTC solution turns red, it cannot be used again. 2. When developing color, all seeds should be immersed in TTC staining solution. 3. After TCC staining, it should be observed immediately and not left for a long time. 4. For your safety and health, please wear laboratory clothes and disposable gloves when operating. Although there are many manufacturers of TRICINE buffering agents, it is important to choose the right one. Currently, Desheng has many cooperative customers, and the quality has received unanimous praise from many customers. The after-sales service is in place, trying to meet customer requirements, and the cost-effectiveness is also high. Welcome to inquire and order!