HEPES-NA biological buffer is a non-volatile zwitterionic chemical buffer, chemical full name N - (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt, CAS number 75277-39-3. Appearance and characteristics: HEPES-NA has a white powder appearance and generally needs to be prepared as a solvent when used, so it has good water solubility and relatively stable chemical properties. Usage: HEPES-NA has a low toxic effect on cultured cells, so it is commonly used as a buffer for cell culture research; It can be used as a buffer in the phosphorylation detection process of permeable fibroblasts; It can be used in combination with HEPES buffer and is a commonly used bio buffer formula; HEPES-NA is also an effective inducer of lysosomal biogenesis; It can also be used to form complex dialysis buffers for the determination of free thyroxine in serum through radioimmunoassay. Precautions for use: After preparing HEPES-NA powder into a solution, it is necessary to label it in a timely manner to distinguish it from other solvents; HEPES-NA needs to be stored in a sealed container, in a cool and dry place, and away from oxidants; When impurities or mold appear in HEPES-NA powder or solution, it should be stopped and continued to be used; Avoid direct contact with human skin. If accidentally touching the skin, rinse immediately with plenty of water. If you still feel , seek medical attention as soon as possible; HEPES-NA has a certain harmful effect on water, so unused HEPES-NA solutions need to undergo special treatment and meet relevant requirements before they can be discarded.