Biological buffering agent MES semi sodium salt is a colorless crystalline powder, with the Chinese name being sodium morpholine sulfonate and the chemical name being 2- (N-morpholino) ethanesulfonic acid half sodium salt. It is easily soluble in water and has buffering ability, which can adjust the acidity and alkalinity of the solution and maintain its stability. In biological and molecular biology experiments, MES semi sodium salts are often used to prepare buffer solutions. For example, Tris MES buffer and MES buffer can maintain the normal function of cells and biomolecules due to their moderate pH range. These buffers are widely used in experiments such as cell culture, enzyme reactions, and protein electrophoresis to provide favorable experimental conditions. In addition, MES semi sodium salts are also used as a component of electrophoretic buffer in molecular biology research. Electrophoresis is a commonly used experimental technique for separating and detecting biomolecules such as DNA, RNA, and proteins.