Introduction and Application of Biological Buffer BIS-Tris Propane The Chinese name of BIS-TRIS propane is 1,3-bis (tri (hydroxymethyl) methylamino) propane, with CAS number 64431-96-5. The appearance is a white crystalline powder, similar to TRIS buffering agents, as it is also a derivative of the TRIS series of biological buffering agents. Let's follow the editor to see what its features and applications are. Introduction to Bis Tris Propane Bis Tris propane is a zwitterionic buffer primarily used in biochemistry and molecular biology. Because it has two similar pKa values, with a primary acid dissociation constant Pka1=6.8 and a secondary acid dissociation constant Pka2=9.0, it exhibits a very wide buffering range, approximately between pH 6.3 and 9.5. It is precisely because of its broad buffering capacity that the pH can be as low as 6-7, making it more suitable than Tris buffer for improving the stability and activity of restriction endonucleases. It is also one of the reagents with a wide range of applications in the TRIS series products. Specific applications of BIS-TRIS propane 1. Using it as a buffer in studying the fidelity of DNA polymerase and PCR reactions has shown good results; 2. Used as a buffer in the study of bacterial rhodopsin electrical signals excited by light; 3. Used to maintain the stability of purified farnesyl pyrophosphate from brewing yeast mutant strains; 4. Used for studying enzyme activity in some experiments; 5. Used for crystallization research in proteins; 6. Used as a buffer in other biochemistry and molecular biology. Hubei Xindesheng Material Technology Co., Ltd Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffering agents. Currently, the Bis Tris propane produced has stable performance and good usage effects, and has been sold to multiple customers both domestically and internationally, receiving continuous praise. In addition to Bis Tris propane, we also produce dozens of other biological buffering agents. You have everything you want. If you have a need to purchase biological buffering agents, please feel free to call us for consultation!