1、 Chemical properties of CHES The chemical name of CHES is 2- (N-cyclohexylamino) ethanesulfonic acid, with the molecular formula C4H10N2O4S and a relative molecular weight of 182.2 g/mol. It is a white crystalline powder that is easily soluble in water at room temperature and has a relatively stable pH value. CHES buffer exhibits good buffering ability in the pH range of 9.3 to 9.7, and is therefore widely used in experiments under alkaline conditions. 2、 The purpose of CHES CHES buffer has a wide range of applications in biochemistry and molecular biology research. It can be used as a buffer system in experiments such as cell culture, protein purification, enzyme activity determination, and nucleic acid electrophoresis. Due to its strong buffering ability near pH 9.5, CHES is widely used to study the reaction mechanism and structure of catalytic alkaline enzymes. 3、 Advantages of CHES CHES buffer has multiple advantages, making it highly favored in biological experiments. Firstly, CHES can provide a wide buffer range, particularly suitable for experiments that require high pH values. Secondly, it does not interact with cell components under physiological conditions and does not produce toxicity or adverse effects on organisms, which is conducive to cell survival and stable cell culture. In addition, its pH value is relatively stable and can maintain relatively constant pH conditions in the experiment, ensuring the accuracy and repeatability of the experiment.