The color developing substrate ADOS is a raw material used for color development in a biochemical reagent kit, with CAS number 82692-96-4. It can react with 4-AAP in the presence of peroxidase to produce a color reaction catalyzed by hydrogen peroxide. The color reaction is sensitive and rapid in detection. Here are guidelines for the use of ADOS reagents produced by our company for reference only. Appearance and packaging of ADOS reagents: The packaging box is a foam insulation box, containing blue ice or ice bag, with product instructions and test reports attached. If it is incomplete, please call customer service for an electronic version. The reagent is packaged in a dark brown bottle and appears as a white crystalline powder. If the color deepens, it may have grown bacteria or partially oxidized and cannot be used. After receiving the goods, the built-in ice bag has melted, will it cause the product to deteriorate? This product is stable at room temperature, and low-temperature transportation is to prevent extreme temperature conditions that may occur during transportation. Therefore, when you receive the product, if the ice bag has melted, it will not affect its quality. Please rest assured to use it. If stored for a long time, it is recommended to refrigerate and avoid light. If you want to prepare a solution, it is recommended to use it immediately to avoid oxidation and discoloration in contact with air. Prepare as a solution: The chromogenic substrate ADOS is a highly water-soluble aniline sodium salt derivative modified from traditional chromogenic phenol and aniline. Reagent powder may adhere to the wall of the tube or bottle, allowing the use of a centrifuge for low-speed centrifugation to reduce product loss; The product has good solubility and can be directly dissolved in deionized water; Double distilled water or ultrapure water can be used for experiments with high requirements; When special circumstances require the use of DMSO as a solvent, it is necessary to first check its solubility in DMSO and set the corresponding DMSO concentration control group; In special circumstances, water bath or ultrasonic vibration can be used to assist in dissolution; When the concentration changes too quickly during the dilution process and substances precipitate, ultrasound can be used to dissolve them again. Is the product sterile This product is a powdered reagent that has been frozen and stored to reduce the possibility of bacterial growth in solution based reagents. When preparing a solution, only the operating environment and equipment and instruments need to be kept sterile; If strict sterilization is indeed required, it is recommended to use a bacterial filter and do not sterilize under high temperature and pressure. When ADOS is used as a chromogenic substrate to participate in hydrogen peroxide coupling chromogenic reactions, the participation of peroxidase is required. Therefore, strict pH requirements are required for the reaction system. It is recommended to use a biological buffer produced by Desheng Technology to regulate the reaction; Environmental pH ensures enzyme activity and improves detection sensitivity.