Acridine ester DMAE-NHS has a yellow powder appearance and is a very important chemiluminescent reagent. It has the advantages of mild reaction conditions, good reproducibility, high luminescence efficiency, and strong luminescence intensity, and is widely used in fields such as inorganic organic compounds, environmental monitoring, biological and drug analysis. It can label proteins, peptides, antibodies, nucleic acids, and is also widely used for sensitive detection and diagnosis of various types of diseases. Precautions for the use of acridine ester DMAE-NHS 1. Before labeling, due to the activation of the carboxyl group at the end of the acridine ester by NHS, it is more active. Please use a non proton dry anhydrous solvent to dissolve it; The active site of acridine ester itself is unstable in alkaline and oxidizing environments, so corresponding non strong alkaline and non oxidizing systems should be prepared based on the stability of the labeled substance for processing and preservation. 2. The luminescence process of acridine ester is very brief (the entire process takes less than 2 seconds), and the triggering scheme must include an internal photometer and photon detector; In addition, this product can also use a multifunctional enzyme-linked immunosorbent assay (ELISA) equipped with an automatic sampler for luminescence data collection, minimizing the impact of sampling time on the collected signal. Thermal Stability of Acridine Ester DMAE-NHS The thermal stability of acridine ester DMAE-NHS decreases with increasing pH and temperature. At room temperature, DMAE-NHS is relatively stable in PB buffer solutions with pH 5.8, 7.0, and 8.0. After 16 days, the luminescence activity decreased by 1.6%, 3.6%, and 8.3%, respectively. At 37 ℃, the luminescence activity of DMAE-NHS in PB buffer with pH 5.8, 7.0, and 8.0 decreased by 8.9%, 22%, and 42% after 16 days, respectively. The reason why the luminescence activity of DMAE-NHS in PB buffer decreases with time is due to the occurrence of hydrolysis, which is beneficial in alkaline environments and increases with temperature. Therefore, the hydrolysis rate of DMAE-NHS increases with the increase of solution pH and temperature. Desheng acridine ester DMAE-NHS is a product that is usually in a golden powder state. As a professional acridine ester manufacturer, Desheng has invested a lot of energy in the research and development of acridine esters. Old customers have received continuous praise, while new customers continue to come. The product quality is excellent and the price is favorable. If you are interested in learning, you can call to inquire. Desheng welcomes you to come and inquire and order.