Luminol is a fluorescent substrate widely used in life sciences and clinical research, typically used to detect the activity of proteins, enzymes, and other biological molecules, especially in cell imaging and biofluorescence experiments. However, as it is a chemical, many people are very concerned about whether it will harm our health, especially the safety during use when formulated into a solution. Below, we will introduce to you whether Luminol solution is toxic, in order to help you use it better. 1、 Toxicity issues of Luminol There is some controversy about the toxicity of luminol. Some in vitro experiments have shown that luminol can bind to human serum albumin, indicating that it is not harmful to the human body. Later, animal experiments conducted by ILS in the United States showed that luminol only causes physiological changes and death in experimental animals at high doses, while it has almost no effect at low doses. In addition, Luminol was directly used for clinical treatment in the 1960s, and there was no evidence of toxic side effects at that time, so it can be considered that Luminol itself poses little harm to the human body. However, it should be noted that Luminol reagent does not only contain the component of Luminol, and the risk of use in Luminol reagent not only comes from Luminol itself, but also from other components in its solution. The following are potential risk components: (1) Sodium hydroxide: Sodium hydroxide is a strong alkali with corrosiveness. Its vapor and particulate matter can cause serious irritation to the respiratory tract, causing throat swelling and coughing. In addition, long-term exposure may lead to chronic respiratory problems. (2) Sodium carbonate: Sodium carbonate is irritating to the eyes and mucous membranes, and may cause conjunctivitis and skin allergies. (3) Hydrogen peroxide: Hydrogen peroxide is a strong oxidant with corrosiveness. Inhaling hydrogen peroxide may cause respiratory damage, and swallowing it may cause gastrointestinal discomfort. (4) Sodium perborate: Sodium perborate is corrosive to the eyes and skin, and may cause irritation and burns.