Sequencing Grade Chymotrypsin Animal Origin Free CAS: 9004-07-3 EC: 3.4.21.1 SOURCE Expressed in E. Coli, purified by HPLC. DESCRIPTION Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. MAIN FEATURES Source E.Coli Purified NLT 95% by HPLC analysis Physical form lyophilized powder Specific activity NLT 1500 USP units/mg pro. No Contaminant activity No carboxypeptidase A, or other proteases contaminant. APPLICATION Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel. This sequencing grade enzyme can be used alone or in combination with other proteases to produce. RECOMMEND USAGE To prepare 1-10mg/ml with 1ml 1mM HCl, used within 2 days, or stored below -20℃ after repacked STORAGE AND STABILITY Recommend recombinant Chymotrypsin lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months. After dissolved, it should be stored under -20℃. RELATED PRODUCT: Recombinant Trypsin (sequencing purity), Recombinant Carboxypeptidase B (sequencing purity), V8 (sequencing purity), Recombinant Chymotrypsin (sequencing purity)
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel. This sequencing grade enzyme can be used alone or in combin

CAS NO:9004-07-3

EC NO:232-671-2

Molecular Formula:

Molecular Weight:

Synonyms:;alpha-chymotrypsin fr.bov.pancr.free of;alpha-Chymotrypsin, TLCK treated;A-chymotrypsin type vi;A-chymotrypsin type ii from bovine*pancreas;gamma-chymotrypsin type ii: from*bovine pancreas;A-chymotrypsin sequencing grade;A-chymotrypsin type I-S from bovine*pancreas;A-chymotrypsin from human pancreas;A-chymotrypsin type iv-S from bovine*pancreas;?-Chymotrypsin, USP Grade alpha-Chymotryspsin, Bovine, USP Grade;a-Chymotrypsin, Bovine Pancreas;Chymotrypsin, Human Pancreas;beta-chymotrypsin;Chymotrypsin;Recombinant Chymotrypsin,from E.coli;Sequencing recombinant Chymotrypsin,from E.coli;alpha-Chymotrypsin;Chymotrypsin,pancreatic basic;

Molecular Structure: